Experimental Study on the Treatment of Ulcerative Proctitis in Rats by Combining Baitouweng Decoction with Mesalamine Retention Enema

To study the effect and mechanism of Baitouweng decoction, mesalamine its combination with an enema in treatment ulcerative proctitis rats is objective study. The were divided into seven groups: Control (Normal), model (ulcerative proctitis), mesalamine, lowdose decoction combined medium-dose high-dose group. Except for control group, all induced a 5 % acetic acid solution proctitis. severity was measured by disease activity index score; morphological changes rectal tissue observed hematoxylin eosin staining; expression cytokines tumor necrosis factor alpha, interleukin-1 beta, interleukin-6, interferon‐gamma, interleukin-10 messenger ribonucleic detected reverse transcription-polymerase chain reaction; Western blot analysis signaling pathway proteins nuclear kappa B p65 phosphorylated-nuclear tight junction claudin-1, occludin zonula occludens-1 immunohistochemistry. results revealed that scores each dosing group significantly lower than those After treatment, pro-inflammatory factors interleukin-6 interferon‐gamma inhibited anti-inflammatory up-regulated. In conclusion, had synergistic on action may be to regulate synthesis release inflammatory inhibiting over-activation p65, reducing damage mucosa repairing intercellular structure, thus improving intestinal barrier response.